il 17rb  (R&D Systems)

Journal: JCI Insight

Article Title: IL-17B alleviates the pathogenesis of systemic lupus erythematosus by inhibiting FASN-mediated differentiation of B cells

doi: 10.1172/jci.insight.181906

Figure Lengend Snippet: ( A ) Serum IL-17B levels and ( B ) IL-17RB expression on CD19 + B cells in healthy controls (HC, n = 37), inactive-SLE patients ( n = 16), and active-SLE patients ( n = 36). Correlation of IL-17RB with ( C ) CD86 and ( D ) CD40 on CD19 + B cells in SLE ( n = 52) and HC ( n = 37). Correlation of IL-17RB with levels of ( E ) anti-dsDNA antibody, ( F ) SLEDAI, ( G ) C3 complement, and ( H ) anti-nucleosome antibodies on CD19 + B cells with SLE ( n = 52). ( I ) Serum FASN levels and ( J ) FASN expression on CD19 + B cells in HC ( n = 37), inactive-SLE patients ( n = 16), and active-SLE patients ( n = 36). Correlation of FASN with ( K ) CD86 and ( L ) CD40 on CD19 + B cells in SLE ( n = 52) and HC ( n = 37). Correlation of FASN with levels of ( M ) anti-dsDNA antibodies, ( N ) SLEDAI, ( O ) C3 complement, and ( P ) anti-nucleosome antibodies on CD19 + B cells in SLE ( n = 52). ( Q ) PCA dot plot, ( R ) volcano plot, ( S ) heatmap, and ( T and U ) GSEA plots were generated based on the lipid profiles of peripheral blood serum from SLE patients ( n = 40) and HC ( n = 40). The data are shown as the mean ± SEM and are representative of 3 independent experiments. * P < 0.05, *** P < 0.001, **** P < 0.0001 by 1-way ANOVA with Tukey’s multiple-comparison test ( A , B , I , and J ). Correlation coefficients were calculated using linear regression analysis. NS, P > 0.05.

Article Snippet: APC-labeled anti–human IL-17RB (catalog FAB1207A) was purchased from R&D Systems, and APC-labeled anti–human FASN (catalog ab223965) was purchased from Abcam.

Techniques: Expressing, Generated, Comparison

Journal: The Journal of Biological Chemistry

Article Title: Interleukin-25 (IL-25) has a protective role in atherosclerosis development in the aortic arch in mice

doi: 10.1074/jbc.RA117.000292

Figure Lengend Snippet: IL-25 deficiency increases splenic Th1 cells and decreases Tregs and ILC2s. Shown are gating strategies (the graphs show the gating strategy in one Apoe−/−/IL-25−/− mouse) to identify immune cells in the spleen with flow cytometry and the frequencies in the splenic cells. In a and b, the same CD3+CD4+ flow cytometry plot is shown, because it is the same sample from one representative mouse that has been stained for both INF-γ and IL-5. a, to identify Th1 cells, leukocytes were gated as CD3+CD4+IFN-γ+ T cells in the CD3+CD4+ population. Shown is the frequency of Th1 cells in the spleen of Apoe−/− and Apoe−/−/IL-25−/− mice. b, to identify Th2 cells, leukocytes were gated as CD3+CD4+IL-5+ T cells in the CD3+CD4+ population. Shown is the frequency of Th2 cells in the spleen of Apoe−/− and Apoe−/−/IL-25−/− mice. c, to identify Th17 cells, leukocytes were gated as CD3+CD4+IL-17+ T cells in the CD3+CD4+ population. Shown is the frequency of Th17 cells in the spleen of Apoe−/− and Apoe−/−/IL-25−/− mice. d, to identify Tregs, leukocytes were gated as CD3+CD4+CD25+FoxP3+ T cells in the CD3+CD4+ population. Shown is the frequency of Tregs in the spleen of Apoe−/− and Apoe−/−/IL-25−/− mice. e, to identify ILC2s, leukocytes were gated as CD45+, lineage-negative cells (Lin−) expressing IL-17RB, intermediate (int) IL-7ra, and ICOS in the CD45+ population. Shown is the frequency of ILC2s in the spleen of Apoe−/− and Apoe−/−/IL-25−/− mice. Mann–Whitney test was used; n = 10–19 for Apoe−/− and n = 22 for Apoe−/−/IL-25−/− mice; *, p = 0.022; ****, p < 0.0001. Each dot represents one mouse, and the bars show the mean value ± S.D.

Article Snippet: The enriched fraction of cells was next stained with fluorochrome-conjugated antibodies, such as Lin-Streptavidin PE/Cy7, ICOS-PB (clone C398.4A), CD45-APC/Cy7 (clone 30-F11), IL-7ra-FITC (clone SB/199), and IL-17RB-APC (clone 752101, R&D Systems, Abingdon, UK) and analyzed with the use of flow cytometry.

Techniques: Flow Cytometry, Staining, Expressing, MANN-WHITNEY